| Crew: 6 | CDR - Ronald J. Grabe |
| Plt - Brian J. Duffy (IV 1) | |
| MS 1 - G. David Low (PLC & EV 1) | |
| MS 2 - Nancy J. Sherlock | |
| MS 3 - Peter J. K. Wisoff (EV 2) | |
| MS 4 - Janice E. Voss | |
| Launch | 21 June 1993 @ 0907:22.047 EDT |
| Orbital incl: | 28.46 |
| Max alt: | 481.2 km. |
| Orbits: | 155 |
| Dur: | 9:23:44:54 |
| Landing: | 1 July 1993 @ 0852:16 EDT |
| Launch Window: | 1 hour, 11 minutes (9:07 - 10:18 am EDT) |
| TAL Sites: | Banjul, Gambia (Alternate: Moron, Spain) |
| AOA site: | Edwards AFB (Alternates: KSC and WS) |
| Primary landing site: | KSC/SLF (Scheduled: 8:44 am EDT, 29 June) |
| Landing Speed: | 200 knots (230 mph) |
| Landing rollout: | 9,946' from main gear touchdown |
| Vehicle weight @ liftoff: | 4,516,459 lbs |
| Orbiter weight @ liftoff: | 252,359 lbs |
| Orbiter weight @ landing: | 224,490 lbs |
| Cargo mass approx.: | 19,691 lbs |
| Landing Weight: | 28,925 lbs |
| Special mission kits: | 3 EMU's, Repackaged Galley, teleprinter, TAGS, Keel TV camera, a high intensity searchlight, TEAC Hi-8 VCR, EDO Toilet, 6 laptops, related EVA hardware and RMS-303 |
| SPACEHAB-01 - Space Habitat Module, Flight Unit-1 | 22 expirements. Total mass - 9,628 lbs |
| SHOOT - On MPESS, mass - 3,570 lbs | Superfluid Helium On-Orbit Transfer Demonstration |
| GAS Bridge Assembly - mass: 5,652 lbs | 10 GAS cans including CONCAP-IV (Preparation of Non-Linear Optical Organic Materials in Microgravity) - see STS-46 |
| EURECA-1R - mass: 9,800 lbs | Retrieval (see STS-46) |
| EVA-1 - 25 June (Low & Wisoff) | Duration - 5 hours, 40 minutes |
| Mid-deck | SAREX-II 'Configuration C' call sign - Duffy (N5WQW (only one member), FARE II (see STS-53) and AMOS (see STS-29) |
| SSME's: | 2019 (1) - 12th use, 2034 (2) - 2nd use, 2017 (3) - 8th use. At Tower Clear 104% |
| OMS Pods/Engines: | LP-03/105 - Pod 15th use, Motor 13th use & RP-04/108 - Pod 11th use, Motor 15th use |
| ET: | ET-58 (LWT-51) |
| SRB's: | BI-059 (RSRM-32) - Set successfully recovered |
| 30 November 1992 | ET-58 arrives on dock KSC |
| 19 January 1993 | OV-105 returns to OPF-1 following Mission-53/STS-54 |
| 8 March 1993 | ET-58/SRB mate in VAB High Bay 3 |
| 14 March 1993 | SPACEHAB-1 & GBA were installed into the orbiter |
| 24 March 1993 | OV-105 rollover from OPF-1 to VAB High Bay 3 for stacking the same day |
| 28 April 1993 | Stack rollout to Pad 39B |
| 30 April 1993 | SHOOT payload was installed into the orbiter while on the pad |
| 14 May 1993 | TCDT countdown dress rehearsal conduted with crew aboard |
| 2 June 1993 | ME-2 turbo pump was removed on the pad and reinstalled on 9 June |
| 17 June 1993 | "Call to stations" 43 hr countdown started at 2:30 am EDT and astronaut arrival at KSC |
| 21 June 1993 | Launch |
| 1 July 1993 | Landing at KSC/SLF. OV-105 returned to the OPF the same day |